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Select Service	Service Description	Fee Structure*

	1. Deposit of Bacteria/Archaea/Fungi/Plasmid	0
	2. Deposit of Genetically Modified Microorganisms	0
	3. Deposit of Antimicrobial Resistant Isolates	0
	4. Deposit of DNA/Proteins	0
	5. Deposit in Bulk (More than 50 Cultures)	0
IDA deposit under Budapest Treaty
	1. Deposit of Bacteria, fungi, yeast and plasmids belonging to Hazard Group 1 and 2 only
	(a) Storage under rule 12.1 (a)(i)	20,000
	(b) Conversion of deposit	20,000
	(c) Extension of duration of storage beyond that provided by Rule 9 (per year)	2,000
	(d) Issue of viability statement on the basis of test	3,000
	(e) Issue of viability statement on the basis of last viability test	1,000
	(f) Furnishing of samples	3,000
	(g) Communication of information under Rule 7.6	1,000
	(h) Attestation referred to in Rule 8.2	1,000
	2. Deposit of Cell lines	50,000
Safe Deposit
	1. Deposit of Bacteria, fungi, yeast and plasmids belonging to Hazard Group 1 and 2 only	10,000 For the first year only (including Rs. 5000/- for ten freeze-dried ampoules). Fee for subsequent years is ` 5000/- per annum.

	1. Freeze dried culture (ampoules)	1,200
	2. Active Cultures on slants/stabs	1,800

A) Identification by rRNA/ITS DNA sequencing
	1. Sequencing of 16S rRNA gene (~700bp)	1,500
	2. Sequencing of 16S rRNA gene (~1200 bp)	2,100
	3. Sequencing of 18S rRNA gene /ITS/D1-D2 (~700bp)	1,700
	4. Sequencing of 18S rRNA gene (~1200 bp)	2,600
B) Molecular & Chemotaxonomic Characterization
	1. DNA G+C Content (mol%, melting temperature based)	8,000
	2. DNA-DNA Hybridisation (per pair)	17,000
	3. MALDI-TOF MS based biotyping	550
	4. Whole-cell Fatty Acid Methyl Ester (FAME) analysis by GC	9,000
	5. Analysis of polar lipids	7,000
	6. Phylogenetic analysis	2,500
C) Phenotypic Characterisation
	1. Scanning Electron Microscopy	2,000
	2. Conventional Characterization
	(a) Bacteria	4,000
	(b) Mycelial fungi Currently Unavailable	4,000
	(c) Yeasts (Morphological & API-AUX C or API Candida) Currently Unavailable	7,000
	3. Analytical Profile Index (API)-based Phenotypic charaterization of Bacteria
	(a) API 20 NE	2,500
	(b) API 20 E	2,100
	(c) API 50 CH	4,500
	(d) API 50 CHL	4,500
	(e) API ZYM	2,500
	(f) API 20 STAPH	2,500
	(g) API 20 STREP	2,500
	(h) API Coryne	2,000
	4. BIOLOG-based Phenotypic charaterization
	(a) BIOLOG GENIII Gram Positive/Negative	3,200
	(b) BIOLOG Fungi	3,600
	(c) BIOLOG Yeast	3,100
	(d) BIOLOG Anaerobic Bacteria	3,500
	(e) Community Level Physiological Profile (CLPP) using BIOLOG ECO Plate	5,500
	(f) CLPP using BIOLOG ECO Plate with analysis of results	7,000
	5. VITEK-based Phenotypic charaterization
	(a) VITEK using GP/GN/YST/BCLcards	1,200
	(b) VITEK using ANC/CBC cards	1,700
	(c) VITEK-based Antimicrobial Susceptibility Testing (AST)	1,400
D) Identification by Sequencing + Conventional Phenotyping
	1. Bacteria (~700 bp Sequence + Phenotypic)	5,500
	2. Bacteria (~1200 bp Sequence + Phenotypic)	6,100
	3. Mycelial fungi (~700 bp Sequence + Phenotypic) Currently Unavailable	5,500
	4. Yeasts (~700 bp Sequence + Phenotypic) Currently Unavailable	8,500

	1. Microbial Genome Sequencing Currently Unavailable	30,000
	2. Microbial Genome Sequencing + bioinformatics for novel taxa Currently Unavailable	35,000
	3. Microbial Genome Sequencing + Advanced bioinformatics Currently Unavailable	40,000
	4. Amplicon Sequencing for less than 50 Samples (16S rRNA or ITS)	10,000
	5. Amplicon Sequencing for more than 50 Samples (16S rRNA or ITS)	8,500
	6. Standard bioinformatics analyses of amplicon data (rRNA or ITS) Currently Unavailable	3,000
	7. Advanced bioinformatics analyses of amplicon data (rRNA or ITS) Currently Unavailable	5,000

A) Purification of Microbial Cultures
	1. Bacterial culture	5,000
	2. Fungal culture Currently Unavailable	4,000
B) Freeze-drying of Microbial Cultures
	1. Batch of 10 freeze-dried ampoules per culture	11,000
	1. Bulk Freeze-drying (upto 50 g pelleted microbial culture, no ampoules)	4,000
C) Cycle Sequencing of PCR Products
	1. Purified PCR products (~700 bp)	700
	2. Un-purified PCR Products (~700 bp)	1,200
D) Microbial Testing of Food Products
	1. All food categories except those mentioned at serial No. 2(a) and 2(b) below. Currently Unavailable	5000
	2. (a) Foods for Infant Nutrition Currently Unavailable I. Infant Milk Food II. Infant Formula III. Milk cereal based. Complementary Foods IV. Processed cereal based complementary Foods (b) Packaged drinking water and Mineral Water	12,000
E) Other Services
	1. Genomic extraction (~50ug)	4,000
	2. MALDI-TOF MS (MSP creation)	1,500
	3. Phytoplasma testing PCR	2,600

Please contact service co-ordinator ([email protected]) in advance for more than 10 deposits at a time.
For Supply of Cultures additional GST will be applicable @ 12% and for all other services GST will be applicable at 18%
GST Number - 27AAATN0848B1ZW
SAC Code - 998393
Additional 200/- as shipping and Handling Charges. REQUESTS FOR INTERNATIONAL SHIPMENTS from investigators working outside India will be charged at actual and follow a different procedure as mentioned here.

Demand Draft should be drawn in favour of 'The Director,NCCS, Pune', payable at Pune.
Electronic money transfer to the below-mentioned account:
Account Name: Director, NCCS
Name of the Bank: Bank of India
Branch Address: Pashan Road, Plot No. 5, Bhuvaneshwar Co. Op. Society,
‘Ashirwad’, Opp. Abhimanshri Society, Pashan Road, Pune 411008
Account No.: 05140110004912
IFSC Code: BKID0000516
Swift Code: 998393

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